Intranasally administered whole virion inactivated vaccine against clade 2.3.4.4b H5N1 influenza virus with optimized antigen and increased cross-protection

Table 1 Genome constellation and 50% egg infectious dose titers of Recombinant H5N1 viruses in embryonated chicken eggs (ECEs)

Recombinant virus	HA	NA	PB2	PB1	PA	NP	M	NS	EID₅₀/mL^a
22W_PR8	22 W HA^b	22 W NA	PR8	PR8	PR8	PR8	PR8	PR8	8.91 ± 0.14
22W_MVV	22 W HA	22 W NA	310-MVV^c	PR8	PR8	PR8	PR8	PR8	9.91 ± 0.14 ***
22W_KY	22 W HA-R90K H110Y^b	22 W NA	310-MVV	PR8	PR8	PR8	PR8	PR8	9.91 ± 0.14 ***

^a50% egg infectious dose (EID₅₀/ml) of recombinant viruses harvested after inoculating 100 EID₅₀ of each recombinant virus into 10-day-old SPF embryonated chicken eggs. The data are presented as the mean ± standard deviation (SD) of three independent experiments
^bThe HA cleavage site was attenuated from RERRRKR to ASGR
^cPB2 gene of A/chicken/Korea/01310/2001 (01310, H9N2) virus with I66M, I109V and I133V mutations
^*Significantly different from the virus titer of 22W_PR8 (***p < 0.001), Statistical significance was calculated by one-way ANOVA with Tukey’s multiple comparisons test

ISSN: 1743-422X