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Fig. 2 | Virology Journal

Fig. 2

From: Intranasally administered whole virion inactivated vaccine against clade 2.3.4.4b H5N1 influenza virus with optimized antigen and increased cross-protection

Fig. 2

Heat stability of the recombinant and inactivated H5N1 strains. Each recombinant virus or inactivated virus was diluted to 32 HAU and incubated at 52 °C for 0.5, 1, 1.5, 2, 3, or 4 h. After heat treatment, the HA titer of each virus was measured and recorded. The HA titers were plotted over time after heat treatment for three sets of comparisons: (a) among 22W_MVV, 21W_MVV, and recombinant viruses with individual HA mutations (R90K, A93T, S125N, and N193D) and (b) among 22W_MVV, 22W_R90K, 22W_H110Y, and 22W_KY and (c) among formaldehyde (F/A)-inactivated 22W_MVV and 22W_KY, binary ethylenimine (BEI)-inactivated 22W_MVV and 22W_KY, and β-propiolactone (BPL)-inactivated 22W_MVV and 22W_KY. Data for each HA titer data are presented as the mean ± standard deviation (SD) of two independent triplicate experiments

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Virology Journal

ISSN: 1743-422X