Fig. 4
From: Inhibitory effect of Alantolactone against varicella-zoster virus in vitro
ALT inhibited VZV-induced IL6 expression in WI-38 cells. (A) Venn graph. Differentially expressed genes (DEGs) and overlapped DEGs in Mock, DMSO + VZV and ALT + VZV group. (B) Pathways linked to inflammation were revealed via KEGG enrichment analysis of overlapped genes. (C) PPI networks identified IL-6 as a hub gene. (D) The inhibitory effect of ALT on IL-6 transcriptional level in VZV-infected WI-38 cells. WI-38 cells were infected with VZV at MOI of 0.04 and treated with or without ALT (10µM). Total RNA was isolated at 3dpi and subjected to qPCR analysis (n = 3). (E) WI-38 cells were inoculated with VZV at MOI of 0.04 and treated with either DMSO or ALT at 10µM. At 0, 2 or 3 days after infection, samples were harvested to analyze the protein levels of IL-6 using Western blotting. The experiment had been repeated three times and one of the typical results was presented. P values were calculated with one-way ANOVA. ( ****P < 0.0001, ns means no significance)