Fig. 2

Characterization of oncolytic viruses on cytokine expression and the regulation of MHC expression in tumor cells. A-F Expression of GM-CSF, IFN-γ and IL15Rα/IL15 fusion protein. 4T1 cells were infected with multiple strains of oncolytic HSV-1 at MOI = 1 for 24 h (A, B, C) or 48 h (D, E, F). Concentrations of indicated cytokines in supernatants were measured using conventional ELISA kits. Data are presented as mean ± SEM (n = 3), and statistical significance was calculated by one-way ANOVA followed by Bonferroni’s multiple comparisons test. **p < 0.01, ***p < 0.001, ****p < 0.0001. G-H MHCI and MHCII expression in 4T1 cells infected with various oncolytic HSV-1 strains. 4T1 cells were infected with oncolytic HSV-1 for 24 h at MOI = 1 then stained with fluorescent antibody against MHCI (G) or MHCII (H) and analyzed by flowcytometry. MFI, mean fluorescence intensity. Data are presented as mean ± SEM (n = 4), and statistical significance was calculated by one-way ANOVA followed by Bonferroni’s multiple comparisons test. ****p < 0.0001