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Table 1 Fluorescence lifetime values and percentage of FRET for FRET-FLIM experiments with MPJSBWMV:GFP in the presence of MPJSBWMV:RFP or PDLP1:RFP. The table shows fluorescence lifetime values for MPJSBWMV:GFP expressed alone or in the presence of MPJSBWMV:RFP or PDLP1:RFP. Lifetime values in ns, SD standard deviation in ns, N number of replicates, n the number of single measurements used to calculate the average. FRET-efficiency was calculated as FRET% and T-test (p-value 0.05) used to calculate statistical significance

From: Studies on the Japanese soil-borne wheat mosaic virus movement protein highlight its ability to bind plant RNA

Proteins

Localization

N

n

Lifetime [ns]

SD [ns]

% FRET

T-test

MPJSBWMV:GFP

PD

3

189

2.480

0.078

-

-

MPJSBWMV:GFP + MPJSBWMV:RFP

PD

3

339

2.005

0.126

19.15

2.2E-190

MPJSBWMV:GFP + PDLP1:RFP

PD

3

141

2.405

0.083

3.02

8E-16