Fig. 4

MPJSBWMV can bind RNA1JSBWMV and RNA2JSBWMV. N. benthamiana leaf crude extracts expressing MPJSBWMV and GFP after agro-inoculation were used to analyze the binding of these proteins to (A) RNA1JSBWMV, (B) RNA2JSBWMV and parts of the viral RNA with and without the tRNA-like structure, respectively. a significantly different to GFP control, b, significantly different to MPJSBWMV:GFP + RNA2JSBWMV. C binding affinity of MPJSBWMV:GFP purified from E. coli to RNA2JSBWMV with and without plant extract from N. benthamiana. a, significantly different to MP.JSBWMV:GFP purified from bacteria without plant extract. A-C Statistical analysis was made with Tukey test (p-value < 0.05). Binding curves are presented in Fig. S2