Identification of RC3H1 as antiviral host factor binding to the non-structural protein 1 of Influenza A virus via a 3-stage computational pipeline and cell-based analysis

Table 1 Top 7 HVPPI scorers of C3H-ZF proteins predicted to bind NS1 of PR8

Gene	Accession Number¹	UniProtID (Mouse)	UniProtID (Human)	HVPPI Score²	Number of ZF motifs³	Protein length (amino acids)	MW (kDa)	Subcellular Localization³	Co-IP Result⁴
CPSF30	NM_006693.4	Q8BQZ5	O95639	0.747	5	269	30	Nucleus	Positive
ZC3H15	NM_018471	Q3TIV5	Q8WU90	0.639	2	426	49	Cytoplasm, Nucleus	Negative
PPP1R10	NM_002714.4	Q80W00	Q96QC0	0.475	1	940	99.07	Nucleus	Negative
ZC3H8	NM_032494.3	Q9JJ48	Q8N5P1	0.353	3	291	34	Nucleus	Positive
MKRN1	NM_013446.4	Q9QXP6	Q9UHC7	0.332	4	482	53.4	Cytosol	Positive
ZC3H18	NM_001294340.2	Q0P678	Q86VM9	0.307	1	953	109	Nucleus	Positive
RC3H1	NM_172071	Q4VGL6	Q5TC82	0.294	1	1133	126	Cytoplasm, P-body, Cytoplasmic granule	Positive

¹ Accession number of gene retrieved from Genbank
² Human-virus PPI probability score calculated using HVPPI web server
³ Number of ZF motifs and subcellular localization of C3H ZF proteins retrieved from Uniprot and QuickGO
⁴ NS1- C3H-ZF protein interactions as determined by co-IP in this study

ISSN: 1743-422X