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Fig. 4 | Virology Journal

Fig. 4

From: Identification of RC3H1 as antiviral host factor binding to the non-structural protein 1 of Influenza A virus via a 3-stage computational pipeline and cell-based analysis

Fig. 4

H1299 cells were either mock or infected with PR8 at MOI of 5. At 24 hpi, the cells were harvested and subjected to subcellular fractionation. Fractionated cell lysate was subjected to WB analysis. (A) WB analysis showing the expression of NS1, RC3H1 and MKRN1 in cytosol and nuclear fractions in mock- or PR8-infected cell. GAPDH and Lamin A were used as markers for cytosol and nuclear fractions respectively. Densitometry analysis was performed to quantify protein expression. The relative levels of (B) RC3H1 and (C) MKRN1 in different subcellular fractions were plotted after normalization against total expression in both fractions. Data represented average of relative level ± s.d. of three independent experiments and p value was calculated using two-way ANOVA followed by Šídák’s multiple comparisons test. (*p < 0.05)

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Virology Journal

ISSN: 1743-422X