Fig. 1
Overview of the computational protocol. In stage (i), SCOP domains were assigned to mouse and viral protein sequences (shown as blue and pink lines, respectively) using SUPERFAMILY2.0. In stage (ii), DISPOT was used to identify highly likely domain-domain interactions based on probabilities (Pij) derived using statistical potentials. The C3H ZF domain in mouse proteins and NS1 effector domain in IAV viral proteins was identified as a significant interaction (indicated with an orange rectangle). In stage (iii), an additional evaluation was performed to refine the candidate pairs that are likely to be interacting. First, the mouse sequences with C3H-ZF domains were converted to human sequences (shown in purple) by homologous assignment. Next pairwise interaction prediction (between human and PR8 NS1 viral sequences) was performed with the Human-Virus PPI server to obtain a ranked probability list identifying 21 likely pairs. Of these, a final 7 were shortlisted for wetbench experimental evaluation. Logos of web tools in the figure were obtained from their respective webpages