Fig. 4
From: Monoclonal antibody and B-cell epitope mapping of the VP7 protein in bluetongue virus

Fine-mapping of the epitopes with mAbs. Primary (A), secondary (B), and tertiary (C) VP7 fusion proteins truncated by the prokaryotic expression were screened to show the antigenic epitopes of the six mAbs by western blotting. The empty vector pGEX-6p-1-GST and full-length VP7 were used as the negative and positive controls, respectively