Skip to main content
Fig. 3 | Virology Journal

Fig. 3

From: Monoclonal antibody and B-cell epitope mapping of the VP7 protein in bluetongue virus

Fig. 3

Characterization of the mAbs. A The reactivity of mAbs was analyzed with indirect ELISA. The dotted line is 2.1-fold that of the negative control. B The reactivity of monoclonal antibodies was analyzed using western blotting. Lane1: BHK-21 cells infected with BTV-1; Lane 2: Recombinant VP7. C Immunofluorescence analysis of the mAbs. BHK-21 cells infected with BTV-1 were subjected to immunofluorescence assay at 24 h. Alexa Fluor488-conjugated goat anti-mouse IgG was used as a secondary antibody. D Isotype identification of mAbs

Back to article page

Virology Journal

ISSN: 1743-422X