Fig. 2
From: Monoclonal antibody and B-cell epitope mapping of the VP7 protein in bluetongue virus
Expression, purification, and identification of recombinant BTV VP7. A SDS-PAGE identification of the expression of VP7. Lane 1: Uninduced bacteria; lanes 2–5: Bacteria induced for 2, 4, 6, and 8 h. B Solubility analysis of recombinant VP7. Lane 1: VP7 in supernatant after ultra-sonic treatment; lane 2: VP7 in precipitate after ultra-sonic treatment. C Purified VP7 verified by SDS-PAGE. Recombinant VP7 was identified by anti-6 × His tag mAb (D) and rabbit anti-BTV-1 positive serum (E)