Fig. 2
From: HPV16 E7 inhibits HBD2 expression by down-regulation of ASK1-p38 MAPK pathway in cervical cancer
HPV16 E6/E7 down-regulated HBD2 in cervical cancer cells. (A, B) SiHa and CaSki cells were transfected with 50 nM HPV16 E6/E7 siRNA or random control RNA sequence, respectively. The tranfection efficiency was determined with E6/E7 mRNA levels measured by RT-qPCR. HBD2 mRNA and protein levels in both cells were assessed 36 h after 50 nM of either random control RNA sequence or HPV16 E7 (C, D) or E6 (E, F) siRNA sequences. After C33A (G) and CaCo2 (H) cells were transfected with 3 µg pcDNA3.1(-)-3FLAG-HPV16 E7 or empty plasmid for 48 h, E7 protein levels was analyzed with Western blot, and HBD2 mRNA and protein levels were determined using RT-qPCR and ELISA, respectively. The data was analyzed with ANOVA to show the differences in all groups and t-tests were used for assessing the significance between any two groups. *P < 0.05, ***P < 0.001, ****P < 0.0001