Fig. 7

ISGs mRNA expression in isolated first trimester placental trophoblasts. Trophoblasts isolated from first trimester placental samples were (A and B) infected with MOI-5 ZIKV or (C and D) stimulated with 1 µg/ml poly(I: C) and RNA collected at 24 h and 48 h (n = 3 patients). qRT-PCR was then used to detect mRNA for antiviral ISGs. Data are normalised to the housekeeping gene RPLP0 and expressed as a fold-change relative to the mock-infected control at each timepoint (data are means ± SE). Statistical analysis was performed on log transformed data at each time point using multiple unpaired T test for each gene