Fig. 6

Interferon mRNA expression in isolated first trimester placental trophoblasts. Trophoblasts isolated from first trimester placental samples were (A and B) infected with ZIKV MOI-5 or (C and D) stimulated with 1 µg/ml poly(I: C) and RNA collected at 24 h and 48 h (n = 3 patient samples in duplicate). qRT-PCR was then used to detect IFNβ, IFNλ1 and IFNλ2/3 mRNA. Data are normalised to the housekeeping gene RPLP0 and expressed as a fold-change relative to the mock-infected control at each timepoint (data are means ± SE). Statistical analysis was performed on log transformed data at each time point using multiple unpaired T test for each gene