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Fig. 2 | Virology Journal

Fig. 2

From: The first trimester human placenta responds to Zika virus infection inducing an interferon (IFN) and antiviral interferon stimulated gene (ISG) response

Fig. 2

Zika virus infects and replicates in isolated first trimester placental trophoblasts. (A) Trophoblasts from first trimester placental samples were infected with ZIKV then at 24hpi and 48hpi ZIKV genome RNA was quantified using qRT-PCR, including in uninfected control cells. Data are means ± SE, n = 3 patient samples. (B) Infectious ZIKV in CTB culture supernatant was quantified by plaque-assay. Data are means ± SE, n = 2 patient samples in duplicate. Data were log transformed and analysed by Ordinary Two Way ANOVA (*** P < 0.005). (C-D) Isolated trophoblasts were fixed and stained for the CTB marker, cytokeratin-7 (red) and ZIKV-E antigen (green). (E, F) Detection of ZIKV genome dsRNA replication intermediates used anti-dsRNA Ab (red), cytokeratin 7 (green) and nuclei (DAPI). Bars represent 50 μm unless otherwise indicated

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Virology Journal

ISSN: 1743-422X