Fig. 6
Effect of eGFP K181 infection on colon inflammation and the NF-κB signaling pathway in allogeneic skin transplantation mice. 1 × 105 PFU eGFP K181 were inoculated intranasally into allogeneic skin transplantation MyD88(−/−) and MyD88(+/+) BALB/c mice on day 14 posttransplantation. The serum levels of (A) TNF-α, (B) IL-1β, (C) IL-6, (D) IL-8, and (E) IL-12 were measured by ELISA; (F) The expression levels of IKKα, IKKβ, p65, p52, TRIF, MyD88, TRAF6, and TAK1 in colon tissues were measured by Western blot at 5, 9, 14, or 21 d postinfection. (G) The levels of anti-flagellin IgG and anti-LPS IgG in serum and MPO in colon tissues were measured at 28 d postinfection. Data are shown as the mean ± SD of six mice per group. ** P < 0.01, *** P < 0.001 vs. Myd88(+/+)