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Fig. 3 | Virology Journal

Fig. 3

From: Cytomegalovirus infection initiates inflammatory bowel disease by activating a positive MyD88/NF-κB feedback loop in allogeneic skin transplantation mice

Fig. 3

Effect of hMIEP-eGFP K181 infection on colon barrier permeability in allogeneic skin transplantation mice. 1 × 105 PFU hMIEP-eGFP K181 or eGFP K181 were inoculated intranasally into allogeneic skin transplantation BALB/c mice on day 14 posttransplantation. (A) A schematic representation of the assay indicating time points of transplantation and MCMV infections. (B) MCMV infection in colon tissues was confirmed by immunofluorescence with positive eGFP (Scale bar: 50 μm); (C) MCMV DNA and (D) IE1 levels in colon tissues were measured by PCR and Western blot, respectively; (E) Body weight and (F) colon barrier permeability were measured; (G) Necrosis (blue arrow) and inflammatory cell infiltration (red arrow) of colon tissues at 5, 9, 14, or 21 d postinfection (Scale bar: 100 μm). Data are presented as the mean ± SD of six mice per group. *** P < 0.001 vs. eGFP K181

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Virology Journal

ISSN: 1743-422X