Table 2 Primers used for the RT-PCR amplification of the target region in the RdRp gene of orthocoronavirus
From: Detection of avian, murine, bovine, shrew, and bat coronaviruses in wild mammals from Mexico
Primer | Sequence | Length (nt) | Amplicon (bp) | Genus |
|---|---|---|---|---|
Ac767 F Ac767 R | AGTGTGACCGTGCTTTACCT AGCAGGCTTAGGGTGTTTTGT | 20 21 | 767 | Alphacoronavirus |
Bc338F Bc338 R | KCAGGATGGTAATGCTGCTATCA TGCGRGCTCTATTCTTWGCAC | 23 21 | 338 | Betacoronavirus |
Bc743 F Bc743 R | TGTTTTGGGCCTCTTGTTAGG TGAAACATTCTGCCAGTCATAGT | 21 23 | 743 | Betacoronavirus |
Dc255F Dc255 R | CTAAACAGTCAGTCAAGCCCG ACTGTGCATTAATACATCCACCT | 21 23 | 255 | Deltacoronavirus |
Dc473 F Dc473 R | ACTATATGCAAGATGGTGAAGC GTCCCAACCACCATAAAATTTGG | 22 23 | 473 | Deltacoronavirus |
Gc347F Gc347 R | TTATGGCGGTTGGGACAACA CACTCAAAAGACGCGCAACA | 20 20 | 347 | Gammacoronavirus |