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Table 2 Consensus primer sets applied for the amplification of the Egyptian ACoV isolates

From: A full-length S1 gene sequencing of a novel emerged GI-19 and GI-23 lineages of Infectious bronchitis virus currently circulating in chicken flocks in upper Egypt reveals marked genetic diversity and recombination events

Sequencing type of the whole S1 gene

ACoV -forward Primer sequence (5’–3’)

ACoV- reverse Primer sequence (5’–3’)

Product size in (bp)

References

ACoV-HVR1-3

GTK TAC TAC TAC CAR AGT GC

CAG AYT GCT TRC AAC CAC C

79–928

[48]

Full-length

AGTBTCYACACAGTGTTAYAAGCG

GGYCTRWANKSRCTYTGGTAG

1591 bp

[49]

TTAAATCATTTCAGTGTGTTAATAAT

CATAACTAACATAAGGGCAA

1704 bp

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Virology Journal

ISSN: 1743-422X