Fig. 6

Comparative analysis and characterization of the predicted tertiary structure revealed the specific mutations in the S1protein of the novel identified IBV strains (GI-23 and GI-19). Briefly, a three-dimensional structural analysis structure template was downloaded from the PDB database, and the HVRs regions of IBVs GI-23 and GI-19 isolates compared to referential and vaccinal strains; were predicted. The deep red color indicates the localization of specific mutations in the S1 protein of GI-23 and GI-19 isolates. The 3D structure was visualized by PyMOL software