Fig. 4

Detailed phylogenetic tree of ACoV strains within genotype GI-23 based on full S1 gene sequences sequencing comparable to other referential strains deposited in Genbank. The phylogenetic analysis of the S1 gene resulted in establishing three sub-clades of 18 ACoV isolates (indicated as black triangle) located in the GI-23 genotype as follows: (ten isolates clustered as GI-23.2.1, one isolate clustered as GI-23.2.2, and other seven isolates clustered as GI-23.3). The tree was constructed by the Maximum likelihood method with 1,000 bootstrap replicates, using MEGA 7.0 software