Fig. 2

Partial sequencing phylogenetic tree were constructed using the nucleotide sequence alignments of S1 gene of various ACoV strains with other reference strains and Egyptian strains. The phylogenetic analysis of S1 gene revealing that 25 ACoV isolates (indicated by black triangle) located in various genotypes as follow: (fifteen isolates labelled with blue color clustered as GI-23, two isolates labelled with red color clustered as GI-12, one isolate labeled with green colour clustered as GI-1 and the last two isolates labelled with blue color clustered as GI-19). The tree was constructed by the Maximum likelihood method with 1,000 bootstrap replicates, using MEGA 7.0 software