Fig. 2
From: Long-term culture of chicken tracheal organoids for the purpose of avian influenza virus research
Characterization of avian tracheal organoids. A, B Growth of organoids of chicken (A) and duck (B) origin directly after isolation from 1 to 4 days post-seeding (dps). The scale bar indicates 500 µm. C Quantification of organoid section surface areas in mm2 from panels (A, B) with the median indicated by the horizontal bar. D Example of fibroblast contaminated area in passage 1 CTOs. The scale bar represents 200 µm. The insert shows a digitally magnified field. E, F Hematoxylin and eosin staining of CTOs showing thin- and thick-walled morphologies at passage 5 (E) and thin-walled morphologies at passage 15 (F). The scale bar represents 50 µm. G, H Immunofluorescent stainings of passage 5 thick-walled CTOs. Basal cells express p63, in orange (G), also indicated by white arrows. Goblet cells, positive for muc5AC, are indicated in green (H). Hoechst, in blue (G, H), stains nuclei. The scale bar represents 50 µm