Fig. 2

SERINC3 down-regulation function of mutant Nef clones. Substitutions 5L, 8R, 12E and 51T were introduced into Nef consensus C (panel A) and the Nef clone isolated from participant SK68 (panel B). For the clone from participant AS02-0802, which naturally encoded 8R and 12E, we reverted these residues to the consensus 8S and 12G (panel C). Here, the activity of each mutant is shown relative to its parental clone, whose activity is set to 1.0 (for context, the NL4-3-normalized activities of the consensus C, SK68 and AS02-0802 parental Nef sequences were 93.5%, 90.5% and 30.4%, respectively). The ANOVA p values are shown, and asterisks indicate significant differences between the mutants and their respective wild-types: p < 0.05 (*), p < 0.001 (***) after multiple comparisons correction. Bars represent the mean activity of three independent experiments with standard deviations shown