Fig. 1
From: Genetic determinants of HIV-1 subtype C Nef-mediated SERINC3 down-regulation
SERINC3 down-regulation activities of participant-derived Nef clones. A Flow cytometry plots showing SERINC3 expression (y axis) in Nef-transfected cells expressing green fluorescent protein (GFP) (x axis) for the positive control (NL4-3 Nef), negative control (G2A Nef) and participant-derived Nef clones (AS02-0207 B2 and AS02-0184 B2). SERINC3 down-regulation activities (% activity) of participant-derived Nef clones were calculated by normalising the median fluorescence intensity (MFI) of SERINC3 expression in Nef-transfected cells (GFP + subset) to the results obtained for the positive (NL4-3 Nef, 100% activity) and negative (G2A Nef, 0% activity) controls. B The correlation (Spearman’s) between assay replicates is shown. C SERINC3 down-regulation activities did not differ between the HPP, TRAPS and Tshedimoso cohorts. The Kruskal–Wallis p value is shown. Bars indicate the medians with inter-quartile ranges