Fig. 4

Ae inhibits the NF-κB signaling pathway activated by ASFV infection. The expression level of (A) MyD88 protein, (B) phospho-NF-κB p65 protein, and (C) pIκB protein in the ASFV-infected group, ASFV-infected group treated with BAY11-7082 or Ae was detected by western blot. The expression of tubulin was used as a positive control. qPCR was used to detect the changes of (D) IL-1β mRNA levels and (E) IL-8 mRNA levels in the ASFV-infected group, ASFV-infected group treated with BAY11-7082, or Ae at different time points. The mRNA level of GAPDH was used as a positive control. All control cells were normal cultured PAMs. The results of three independent experiments (mean ± SD) were represented by one data. Significant differences that were compared to the control group were indicated by * (P < 0.05), ** (P < 0.01) and *** (P < 0.001)