Fig. 5

Ae promotes apoptosis by inhibiting the NF-κB signaling pathway. A The apoptosis of PAMs in the ASFV infection group, BAY11-7082 or Ae-treated ASFV infection group was detected by flow cytometry, and the apoptosis of induced cells was detected at 3, 12 and 48 h after treatment. Untreated cells served as negative controls. The expression level of B Bcl-2 protein, cleaved-caspase3 protein, and Bax protein in the ASFV infection group, BAY11-7082 or Ae-treated ASFV infection group was detected by western blot. All control cells were normal cultured PAMs. The tubulin expression was used as a positive control