Table 1 Primers used in qRT-PCR assay
Primer | Sequence(5′ → 3′) |
|---|---|
HCoV-229E NP Sense | CGCAAGAATTCAGAACCAGAG |
HCoV-229E NP Antisense | GGCAGTCAGGTTCTTCAACAA |
GAPDH (human) Sense | CTCTGGAAAGCTGTGGCGTGATG |
GAPDH (human) Antisense | ATGCCAGTGAGCTTCCCGTTCAG |
HCoV-OC43 NP Sence | CGATGAGGCTATTCCGACTAGGT |
HCoV-OC43 NP Antisense | CCTTCCTGAGCCTTCAATATAGTAACC |
HCoV-OC43 NP Probe | TAMRA-TCCGCCTGGCACGGTACTCCCT-BHQ2 |
GAPDH (human) Sense | CGGAGTCAACGGATTTGGTCGTAT |
GAPDH (human) Antisense | AGCCTTCTCCATGGTGGTGAAGAC |
GAPDH (human) Probe | TAMRA- CCGTCAAGGCTGAGAACGG -BHQ2 |
IFN-α Sense | CTGTCCTCCATGAGATGATCC |
IFN-α Antisense | CTCATGATTTCTGCTCTGACAACC |
IFN-β Sense | GCTGGAATGAGACTATTGTTGAGA |
IFN-β Antisense | CAGTTTCGGAGGTAACCTGTAAG |