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Table 3 Intra- and inter-assay coefficients of variation of multiplex real-time quantitative PCR

From: A TaqMan probe-based multiplex real-time quantitative pcr for simultaneous detection of kobuvirus, parechovirus B, rosavirus B, and hunnivirus carried by murine rodents and shrews

Target virus

 

intra-group analysis

 

inter-group analysis

  

Cq (means)

SD

CV(%)

 

Cq (means)

SD

CV(%)

Kobuvirus

S7

16.68

0.08

0.48

 

16.42

0.23

1.41

S6

20.25

0.05

0.25

 

20.24

0.22

1.06

S5

24.65

0.08

0.32

 

24.22

0.68

2.80

S4

27.26

0.03

0.11

 

26.97

0.62

2.30

S3

30.76

0.09

0.29

 

30.86

0.34

1.09

S2

34.39

0.61

1.77

 

34.74

0.37

1.07

Parechovirus B

S7

15.81

0.01

0.06

 

15.70

0.11

0.70

S6

19.23

0.04

0.21

 

19.08

0.13

0.69

S5

23.11

0.01

0.04

 

23.03

0.19

0.81

S4

26.67

0.07

0.26

 

26.43

0.27

1.04

S3

30.95

0.05

0.16

 

30.82

0.29

0.95

S2

34.31

0.13

0.38

 

34.39

0.48

1.40

Rosavirus B

S7

12.48

0.05

0.40

 

12.75

0.28

2.16

S6

17.6

0.03

0.17

 

17.79

0.30

1.71

S5

21.9

0.08

0.37

 

21.92

0.54

2.44

S4

25.84

0.10

0.39

 

25.86

0.43

1.64

S3

29.84

0.29

0.97

 

29.89

0.35

1.18

S2

33.47

0.32

0.96

 

33.73

0.29

0.87

Hunnivirus

S7

16.18

0.16

0.99

 

16.21

0.12

0.75

S6

19.59

0.02

0.10

 

19.78

0.17

0.85

S5

22.72

0.04

0.18

 

22.73

0.25

1.08

S4

26.38

0.08

0.30

 

26.46

0.71

2.68

S3

29.64

0.11

0.37

 

29.84

0.21

0.70

S2

32.99

0.07

0.21

 

33.08

0.24

0.73

  1. SD: Standard error
  2. CV: The coefficient of variation