Table 1 Primers and probes for the multiplex real-time quantitative PCR assay
Target virus | Primer/Probe Name | Sequence 5’ -3’ | Length (bp) | |
|---|---|---|---|---|
Kobuvirus | q-Kobu-F | TGTCATCTACGCYACWGAACCC | 197 bp | |
q-Kobu-R | CCAWCACWGGGTGGATGTAGA | |||
Probe-Kobu-F | FAM-TGGACGCTTGTCATCAGGAACAAACCAG-BHQ1 | |||
Parechovirus B | q-PeVB-F | GTGGCTTTCACCTCTRGACAGT | 129 bp | |
q-PeVB-R | GTAATCCGYTGGCACTCAAGTAGC | |||
Probe-PeVB-F | CY5-CATTCCACACCCGCTCCACGGTAGA-BHQ3 | |||
Rosavirus B | q-Rosa-F | ACGCYCTTGCCGAYGTCCTMA | 165 bp | |
q-Rosa-R | TCAAYGARTTGAACACRCTAGTCC | |||
Probe-Rosa-R | Texas Red-CCYGACGGCATGGCRCCATCRAG-BHQ2 | |||
Hunnivirus | q-Hunni-F | AGAGCAARATGTGGACCTGTG | 149 bp | |
q-Hunni-R | TCCGTTTGCCAAAGCAGCTC | |||
Probe-Hunni-F | VIC -CTTTGGGACTGACAACCTGGACCCGAT-BHQ1 | |||