Fig. 3

Expression and processing of artificial primary microRNA from scAnc80. Northern blot hybridization was performed on total RNA extracted from murine liver samples at 1 or 12 months post infection with scAAVs using probes complementary to sequence for guides (a) 5, (b) 8 or (c) 9. The figure shows data from a representative of 3 mice per group. After stripping miR probes from the blots, rehybridization with a probe complementary to U6 small nuclear RNA probe was carried out to confirm equal loading. MW, molecular weight