Fig. 2

Assessment of scAAV activity in culture. a. Assessment of HBV gene expression knockdown by scAAV vectors following transfection of Huh7 cells with psiCHECK-HBx. Data are represented as a mean ratio of Renilla to Firefly luciferase reporter activity. b. Transfection with pCH 9/3901 then transduction with scAAVs was performed before measurement of HBsAg. c-f. Evaluation of HBsAg (c), secreted HBV viral particle equivalents (d, VPEs), intracellular HBV RNA (e) and HBcAg levels in HepG2-hNTCP cells infected with HBV and then transduced with scAAVs. Data shown are relative to control vectors encoding anti-HCV apri-miRs. The means (± SEM) were derived from n = 3–4 samples. The Student’s two-tailed paired t-test or Tukey’s multiple comparison test was used to ascertain statistically significant differences relative to controls. pvalues < 0.05 (*), < 0.001 (**), < 0.0001 (***) were regarded as statistically significant