Skip to main content
Fig. 1 | Virology Journal

Fig. 1

From: Long-term inhibition of Hepatitis B virus gene expression by a primary microrna expressing ancestral adeno-associated viral vector

Fig. 1

Study design. (a) The scAAV vector genome was engineered to carry mono- or polycistronic artificial primary microRNA (apri-miR) sequences under control of a mouse transthyretin receptor (mTTR) promoter. ITR (inverted terminal repeats), int (intron) and TTS (transcription termination signal) are indicated schematically. (b) Engineered scAAV genomes were packaged into AAV2, AAV8 or Anc80L65 capsids. c-f. Upon administration, vectors mediate expression of pri-miRs (c) which are processed in several steps to generate pre-microRNA (pre-miR, d), and miR guides 5, 8 and 9 (e). Produced miRs then target all four HBV transcripts (green, purple and orange arrows shows target sites (f)) (created with Biorender)

Back to article page

Virology Journal

ISSN: 1743-422X