Table 1 Primers used for nested PCR in ORF22 and ORF62
ORF | Primer name | Sequence(5´→3´) | Position * | Amplicon (bp) |
|---|---|---|---|---|
22 | ORF22-W1F | AACAAACGACTCGGGTTTTG | 37,825–37,844 | 652 |
ORF22-W1R | AGTTTAGCCGTTTCGTGTGC | 38,457–38,476 | ||
22 | ORF22F | TAGCATGTCTGGAGGCAATGG | 37,870–37,890 | 359 |
ORF22R | ATCGGCGGATAATAACACAACC | 38,207–38,228 | ||
62 | ORF62-W1F | ATTACTCTGGGAATCGGGGG | 105,607–105,626 | 1908 |
ORF62-W1R | TATAGCATGGCTCCAGAACCC | 107,494–107,514 | ||
62 | ORF62F | GGCCACATTACTCTGGGAAT | 105,601–105,620 | 1793 |
ORF62R | GTTCCGCATGTAGGCGAAG | 107,375–107,393 |