Fig. 1

Both the RNA-binding (NTD) and dimerization (CTD) domains of the N protein contribute to CHK binding. A Schematic of the N protein domain architecture and binding sites of each anti-N Ab used in this study. B, C Anti-N Abs bound to immobilized N reduce N CHK-binding activity. BLI sensorgrams of binding assays showing association and dissociation phases of the interaction between the N protein and 3 positively bound CHKs (upper graphs). Prior to the association phase with CHKs, immobilized N was incubated with B one of each anti-N Ab, or C simultaneously with Ab 2 and Ab 6, up to binding saturation. The dotted line indicates the end of the association phase. The binding response of each CHK to N, in the absence of prior incubation with anti-N Abs, was considered 100% of binding response (lower graphs). Data represent the mean ± SEM (n = 2). One representative assay out of three independent assays performed in duplicate is shown. D Proposed model of biomolecular interaction between N and CHKs, where both the NTD and CTD RNA-binding domain of N act as CHK-binding sites