Table 2 Quantity of ssDNA and target protein in the SELEX process for selection of aptamers against influenza B hemagglutinin
From: Selection and identification of an ssDNA aptamer against influenza B virus hemagglutinin protein
SELEX cycle | Target protein quantity (influenza B HA protein) | Amount of ssDNA | Incubation time (min) | ||
|---|---|---|---|---|---|
µg/mL | ng/µL | nM | Protein coupling | Library screening | |
1 | 575 | 29.15 | 1251.56 | 60 | 60 |
2 | 575 | 39.99 | 1716.4 | 60 | 60 |
3 | 288.75 | 34.43 | 1477.9 | 60 | 60 |
4 | 288.75 | 25.79 | 1106.7 | 50 | 50 |
5 | 256.67 | 24.37 | 1046 | 50 | 50 |
6 | 256.67 | 24.98 | 1072.1 | 45 | 40 |
7 | 128.33 | 23.86 | 1024.3 | 45 | 40 |
8 | 128.33 | 20.82 | 893.6 | 40 | 30 |