Fig. 2

The effect of DVG 2.2 on coronavirus RNA synthesis under different infection conditions. (A) and (C) Diagram depicting the experimental procedure used to examine the effects of DVG 2.2 on coronavirus RNA synthesis, in which DVG 2.2 transfection was performed before (A) or after (C) BCoV infection. (B) and (D) Comparison of the relative amounts of BCoV RNA, IFN-β mRNA and ISG15 mRNA between mock-transfected (inf.) and DVG 2.2-transfected (DVG 2.2) HEK-293T cells infected with BCoV after (B) or before (D) transfection. “Mock” indicates that the HEK-293T cells were mock-transfected and mock-infected. Upper panel: The “Folds/inf.” on the y-axis is presented as relative units of BCoV RNA in DVG 2.2-transfected and BCoV-infected cells compared with the amount of BCoV RNA in mock-transfected and BCoV-infected cells (the amount of BCoV RNA in mock-transfected and BCoV-infected cells (inf.) = 1). Middle and lower panels: The “Folds/mock” on the y-axis is presented as relative units of mRNA compared with the amount of mRNA in mock-transfected and mock-infected cells (the amount of mRNA in mock-transfected and mock-infected cells (mock) = 1). **p < 0.01, ***p < 0.001