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Table 4 Virus propagation in embryonated chicken eggs and cytopathic effect across five passages

From: Isolation, molecular identification, and phylogenetic analysis of infectious bronchitis virus from commercial chicken farms in Mekele and Bishoftu, Ethiopia, 2023–2024

Sample ID

No of

eggs

1st passage

(72 h)

2nd passage

(72 h)

3rd passage

(48 h)

4th passage

(48 h)

5th Passage

(48 h)

Farm 1 (S)

5

Congestion

Haemorrhage

Congestion

Haemorrhage

Stunted Growth

Curling

Curling Deformation

Stunted Growth

Curling

Farm 2 (S)

5

Congestion Haemorrhage

Congestion Bleeding

Stunted Growth

Curling

Curling

Deformation

Stunted Growth

Curling

Farm 6 (T)

5

Congestion Bleeding

Congestion

Bleeding

   

Farm 9 (S)

5

Congestion Bleeding

Congestion Bleeding

   

Farm 10 (S)

5

Congestion

Haemorrhage

Congestion

Haemorrhage

   

Farm 11 (S)

5

Congestion Haemorrhage

Congestion Bleeding

   

Total

30 eggs

     
  1. S represents the swab samples, and T represents the tissue samples