Table 1 Primers and probe sequences for HAV RNA and B19V DNA
ID | Sequence (5’-3’) | Amplicon | Reference |
|---|---|---|---|
HAV | |||
5’NCR (F) | CTGCAGGTTCAGGGTTCTTAAATC | 89 bp | [42] |
5’NCR (R) | GAGAGCCCTGGAAGAAAGAAGA | ||
5’NCR (Probe) | FAM-ACTCATTTTTCACGCTTTCTG | ||
VP1/2A – 1 (F) | CTATTCAGATTGCAAATTAYAAT | 391 bp | [43] |
VP1/2A – 1 (R) | AAYTTCATYATTTCATGCTCCT | ||
VP1/2A – 2 (F) | TATTTGTCTGTYACAGAACAATCAG | 244 bp | |
VP1/2A – 2 (R) | AGGRGGTGGAAGYACTTCATTTGA | ||
B19V | |||
NS1-E1905 (F) | TGCAGATGCCCTCCACCCA | 102 bp | [46] |
NS1-E1987 (R) | GCTGCTTTCACTGAGTTCTTC | ||
NS1 (Probe) | FAM-ACCTCCAAACCACCCCAATTGTCACA | ||
P12 (F) | CAGCCATACCACCACTGGGACA | 563 bp | [47] |
P16 (R) | AGTACATATGGTAAATGACCTGCTG | ||
P13 (F) | GACAAAGAGTATCAGCAAGGAGTG | 476 bp | |
P16 (R) | AGTACATATGGTAAATGACCTGCTG |