Development of a duplex real-time recombinase aided amplification assay for the simultaneous and rapid detection of PCV3 and PCV4

Table 4 Comparison of the duplex real-time RAA with the qPCR assays on clinical samples

Assay		Duplex real-time RAA			Overall agreement	Kappa	p-value
Assay		Positive	Negative	Total	Overall agreement	Kappa	p-value
PCV3 qPCR	Positive	25	1	26	98.33%	0.966	< 0.001
	Negative	0	34	34
	Total	25	35	60
PCV4 qPCR	Positive	8	0	8	100%	1	< 0.001
	Negative	0	52	52
	Total	8	52	60

ISSN: 1743-422X