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Fig. 4 | Virology Journal

Fig. 4

From: Development of a duplex real-time recombinase aided amplification assay for the simultaneous and rapid detection of PCV3 and PCV4

Fig. 4

Screening the optimal primer-probe combination for the duplex real-time RAA for PCV3 and PCV4. Representative amplification results of the duplex real-time RAA assay using 6 primer-probe combinations. Amplification curves in different colors represent different primer-probe combinations. (A) The amplification result of the FAM channel, corresponding to the amplification curves of PCV4; (B) The amplification result for the HEX channel, corresponding to the amplification curves of PCV3; (C) The amplification results of both channels

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