Fig. 4

Screening the optimal primer-probe combination for the duplex real-time RAA for PCV3 and PCV4. Representative amplification results of the duplex real-time RAA assay using 6 primer-probe combinations. Amplification curves in different colors represent different primer-probe combinations. (A) The amplification result of the FAM channel, corresponding to the amplification curves of PCV4; (B) The amplification result for the HEX channel, corresponding to the amplification curves of PCV3; (C) The amplification results of both channels