Effect of eicosapentaenoic acid on innate immune responses in Atlantic salmon cells infected with infectious salmon anemia virus

Table 1 List of primers used in qPCR analysis

Genes	Direction	Sequence 5′ → 3′	Accession number	Amplicon	Melting temp [°C]	References
ef1a	F	CACCACCGGCCATCTGATCTACAA	AF321836	77	58.8	[60]
ef1a	R	TCAGCAGCCTCCTTCTCGAACTTC	AF321836	77	60.6	[60]
18S	F	TGTGCCGCTAGAGGTGAAATT	AJ427629.1	61	54.8	[61]
18S	R	GCAAATGCTTTCGCTTTCG	AJ427629.1	61	52.9	[61]
ifna1	F	CCTGCCATGAAACCTGAGAAGA	AY216594	107	55.4	[59]
ifna1	R	TTTCCTGATGAGCTCCCATGC	AY216594	107	55.1	[59]
isg15	F	ATGGTGCTGATTACGGAGCC	AY926456	151	54.2	[62]
isg15	R	TCTGTTGGTTGGCAGGGACT	AY926456	151	53.9	[62]
mx1/2	F	TGATCGATAAAGTGACTGCATTCA	NM_001123690.1/ NM_001123693.1	80	54.8	[63]
mx1/2	R	TGAGACGAACTCCGCTTTTTCA	NM_001123690.1/ NM_001123693.1	80	55.9	[63]
ifih1	F	GAGAGCCCGTCCAAAGTGAA	XM_014164134	389	53.9	[54]
ifih1	R	TCCTCTGAACTTTCGGCCAC	XM_014164134	389	53.9	[54]
ISAVseg5	F	GAAAGCCCTGCTCTGGC	HQ259675.1	50	51.8	[64]
ISAVseg5	R	TCCTCAAGTCTGCTTCGGGA	HQ259675.1	50	55.2	[64]
ISAVseg6	F	AGGCCAAAAACGGAAATGGA	HQ259676.1	118	51.6	[64]
ISAVseg6	R	CCGTCAGTGCAGTCATTGGTT	HQ259676.1	118	54.9	[64]
ISAVseg7	F	GAAATGGACAGAGACGGCGTATCA	HQ259677.1	124	57.9	[64]
ISAVseg7	R	GCTCAACTCCAGCTCTCTCATTGT	HQ259677.1	124	59.0	[64]

ef1a—elongation factor 1 alpha, 18S—18S ribosomal RNA, ifna1 – interferon alpha-1, isg15—interferon-stimulated gene 15, mx1/2—interferon-induced GTP-binding protein Mx1/2, ifih1—interferon induced with helicase C domain 1, ISAVseg5 – infectious salmon anemia virus gene segment 5, ISAVseg6 – infectious salmon anemia virus gene segment 6, ISAVseg7 – infectious salmon anemia virus gene segment 7

ISSN: 1743-422X