Fig. 2
From: Construction of a Vero cell line expression human KREMEN1 for the development of CVA6 vaccines
Construction of a transfected Vero cell line with stable expressing of KRM1. (A) Schematic of the experimental workflow for constructing a transfected Vero cell line with stable expressing of KRM1. (B) Western blot analysis of RD, Vero, Vero-KRM1_#11 and Vero-KRM1_#26 cells with anti-KRM1 polyclonal antibody. GAPDH was used as the internal reference. (C) The levels of KRM1 mRNA as measured by quantitative real-time RT-PCR. The experiments were performed independently in triplicate. The values are expressed as the mean ± SD. Statistical significance was determined using unpaired Student’s t test. ****p < 0.0001, n.s., not significant