Fig. 2

Development of cytopathogenic effects (CPE) following Canid alphaherpesvirus 1 (CHV) infection in vitro. A Progression of CPE after CHV virus stock infection in the Madin-Darby canine kidney cell line (MDCK) over time (marked in days, hours, minutes, and seconds). B Progression of CPE after CHV virus stock infection in canine adipose-derived mesenchymal stem cells (cAD-MSCs) over time (marked in days, hours, minutes, and seconds). C cAD-MSC negative control (left) and CPE development in six cAD-MSC donors 48 h after infection with the CHV stock (right). D CHV genome copies per mL of cell lysate supernatant for each virus passage in cAD-MSCs (boxplots) and MDCK cells (line graph). Microscopy images were acquired with the Lux 2 live imaging platform (Axion Biosystems) (A, B) or with the microscopic camera Axiocam ER/105/208/HD on Axio Observer D1 (Zeiss, magnification 50-100x, scale bar 200 µm) (C)