Fig. 5
Humoral immunity elicited by CND-1 alone and combined with ODN-39 M, administered by intranasal route in Balb/C mice. Six- to eight-week-old mice were immunized with three doses of each formulation: Group 1: CND-1, Group 2: CND-1 + ODN-39 M, Group 3: PBS. Thirty days after the third immunization, mice were sacrificed. Antibody responses in the serum and BALF were evaluated. a Diagram mouse immunization. Humoral immune response anti-N protein from SARS-CoV-2 measured by ELISA b IgG and c IgG1 and IgG2a in sera, and d IgA in BALFs. Humoral immune response anti-HBcAg measured by ELISA e IgG in sera, and f IgA in BALF. Data of IgG are represented as log10 of the titers. Data from IgA are expressed as O.D492nm. The horizontal bar represents the mean value. The statistical analysis was done by One-Way Anova followed of Tukey’s multiple comparison test. **p < 0.01, ***p < 0.001