Fig. 1
From: No evidence of spread of Linda pestivirus in the wild boar population in Southern Germany
(A) Genome structure of the parental full-length cDNA clone pA/BV and of the newly generated chimeric construct pa/BV_E1E2_LV. The substituted structural proteins E1 and E2 of Linda virus (LindaV) are presented in blue. (B) Immunofluorescence staining of SK6 cells infected with Bungowannah virus (BuPV) expressing the E1 and E2 protein of LindaV (chimeric virus BV_E1E2_LV). Uninfected cells were used as controls. A pan-pesti NS3 specific antibody (anti-NS3), a BuPV specific monoclonal E2 antibody (anti-BV-E2) and a serum of a rabbit immunized with a recombinant LindaV E2 protein (anti-LV-E2) were applied. Scale bars indicate 100 μm. (C) Growth characteristics of the parental BuPV and of the chimeric virus BV_E1E2_LV in SK6 cells