Fig. 7
Induction of HDAC6 expression during SARS-CoV-2 infection facilities disruption of Stress Granules. A A549 cells were transfected with pcDNA3.1-N protein and/or co-transfected with shHDAC6 followed by either tubacin (2.5 µM) or DMSO treatment. Cells were treated with NaAsO2 1 h before harvesting. Co-immunoprecipitation was performed from the cellular lysates in using anti-TIA-1 antibody. Western blot was performed using PABP, N protein, G3BP1, HDAC6 and TIA-1-specific antibodies. B A549 cells were either transfected with pcDNA3.1-N protein or empty vector and either treated with tubacin (2.5 µM) or left untreated. 1 h before fixation cells were treated with NaAsO2 followed by permeabilization and staining with anti-G3BP1 and anti-N protein primary antibody. Secondary staining was performed with Rhodamine-conjugated anti-mouse (for G3BP1) and DyLight™ 488-conjugated anti-rabbit (for N protein) secondary antibodies. DAPI was used for mounting. Imaging was done with a confocal microscope (63× oil immersion) and scale bar was set at 5 μm