Fig. 6

HDAC6 mediates interaction between G3BP1 and SARS-CoV-2 N protein. A A549 cells were transfected with pcDNA3.1-N and co-transfected with either pcDNA-HDAC6-FLAG or shHDAC6 were analysed by co-immunoprecipitation by using anti-G3BP1 antibody. Immunoprecipitates were subjected to western blot analysis using HDAC6 and SARS-CoV-2 N protein-specific antibody. B A549 cells transfected with pcDNA3.1-N plasmid either in presence or absence of tubacin (2.5 μM), followed by co-immunoprecipitation of cellular lysates with anti-SARS-CoV-2 N protein antibody. Immunoblot analysis was performed to check the expression of G3BP1 and HDAC6. C A549 cells were transfected with pcDNA3.1-N and co-transfected with either pcDNA-HDAC6-FLAG or pcDNA-HDAC6.DC-FLAG. Cellular lysates were subjected to co-immunoprecipitation using anti-G3BP1 antibody. Western blot analysis was performed using SARS-CoV-2 N protein and HDAC6-specific antibodies. Input lanes were probed with anti-G3BP1, anti-HDAC6 and anti-SARS-CoV-2 N antibodies. D A549 cells transfected with pcDNA3.1-N and either treated with tubacin (2.5 μM) or treated with DMSO. Cell lysates were subjected to immunoprecipitation using anti-Ac-Lys antibody. Western blot was performed using G3BP1 and HDAC6-specific antibodies