Fig. 3

Increased interaction between G3BP1 and HDAC6 during SARS-CoV-2 infection. A Cellular lysates from A549 cells either infected with SARS-CoV-2 at a MOI of 2 (18–32 hpi) or kept mock-infected were run on SDS-PAGE and protein level expression of acetylated α-tubulin, HDAC6 and NSP13 protein was analysed via western blotting. B SARS-CoV-2-infected (18–32 hpi) or mock-infected lysates were pulled down using HDAC6-specific antibody followed by western blot analysis of the immunoprecipitates by anti-G3BP1, anti-HSP90 and anti-NSP13 antibodies. Inputs were probed with anti-HSP90, anti-G3BP1, anti-HDAC6, anti-NSP13 and β-actin antibodies confirming protein expression. (C) Reciprocal co-immunoprecipitation analysis was carried out in SARS-CoV-2-infected (18–32 hpi) or mock-infected lysates; anti-G3BP1 was used for pull down and immunoblot analysis was performed with HDAC6-specific antibody. Inputs were probed with anti-G3BP1, anti-HDAC6 and β-actin antibodies